Hello, So when I was diagnosed back ten years ago in 2007 my Biopsy did not hurt at all, in fact I was put under for it. I am not sure how it is performed these days but my thought is that its the same, I say that because I have not had one since 2007. So if they put you under you should NOT feel anything at all, just maybe sore afterwards. I am also sure that if you have a biopsy while awake yes you may feel something, that's commend sense.